Composite

Part:BBa_K1065307

Designed by: Caterina Marchioretti   Group: iGEM13_UNITN-Trento   (2013-09-15)

J23100+YF1+FixJ+terminator

This is the intermediate part to build a blue light inducible genetic circuit. The part produces YF1 and FixJ under the control of a strong promoter of the Anderson family BBa_J23100. Two terminators are placed after FixJ.

Note. this part was not yet submitted to the registry but we are going to submitt it soon. Meanwhile, if you are interested in this part please contact iGEM UniTN Trento 2013.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 1590
    Illegal NgoMIV site found at 1684
    Illegal AgeI site found at 319
    Illegal AgeI site found at 1465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1354
    Illegal BsaI.rc site found at 218


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